対訳博論目次
(原文)
Contents
1. Background
1.1 General Introduction - 1
• Importance of stem cells
• Unique properties of all stem cells
1.2 Adult stem cell - 7
• History of Adult stem cell research
• Function of adult stem cells
1.3 Research methods for identifying adult stem cells - 10
• Normal differentiation pathways of adult stem cells.
• Transdifferentiation.
1.4 Pluripotent stem cells - 14
• The similarities and differences between embryonic and adult stem cells
• Embryonic stem cells
A. Establish<→Estalishment> of embryonic stem cells grown in the laboratory
B. Tests for identifying embryonic stem cells
• Induced pluripotent stem cells
1.5 Possibility of existence of adult pluripotent stem cells - 20
• Spore-like stem cells
1.6 Hypothesis of this research - 23
2. Isolation of small cells
2.1 Introduction - 27
2.1.1 Stem cells and small cells - 27
2.1.2 Stem cells and sphere formation - 27
2.2 Experimental<→Experiment> -28
2.2.1 Isolation of small cells - 28
2.2.2 Characterization of small cells - 29
2.3 Results - 30
2.3.1 Sphere forming from isolated cells - 30
2.3.2 Effect of trituration - 30
2.3.3 Culture conditions of small cells - 31
2.3.4 Sphere formation from bone marrow cells - 31
2.3.5 Immature cell marker expression - 32
2.3.6 Expression of embryonic like phenotypes - 33
2.3.7 Quantitative PCR of Pluripotent markers - 34
2.4 Summary of section 2 - 35
2.5 Discussion - 35
2.6 References - 45
3. Characterization of small cells
3.1 Introduction - 48
3.1.1 Differentiation potential of stem cells
3.2 Experimental<→Experiment> - 48
3.2.1 Differentiation potential of cells in vitro - 48
3.2.2 Differentiation potential in vivo - 48
3.3 Results - 49
3.3.1 Differentiation potential of cells in vitro -50
3.3.2 Differentiation potential of cells in vivo - 51
3.4 Summary of section 3 - 51
3.5 Discussion - 51
3.6 Referances - 58
4. Isolation of small cells from TISSUES DERIVED FROM ALL THREE GERM LAYERS
4.1 Introduction - 63
4.2 Experimental<→Experiment> - 63
4.2.1 Isolation of small cells from various tissues - 64
4.2.2 Characterization of isolated small cells - 64
4.2.3 Differentiation potential of sphere forming small cells - 64
4.3 Results - 65
4.3.1 Sphere formation from tissues derived from representative of the three germ layers - 65
4.3.2 Immature gene expression of spheres derived from tissues representative of the three germ layers -66
4.3.3 Differentiation potential of Cells derived from tissues representative of the three germ layers-67
4.3.4 Differentiation potential of cells in vivo - 68
4.4 Summary of section 4 - 69
4.5 Discussion - 69
4.6 References - 76
5. Chimera mice generation from ISOLATED SMALL CELLS
5.1 Introduction - 81
5.1.1 Adult stem cells and chimera mice - 81
5.2 Experimental<→Experiment> - 81
5.2.1 Chimera mice generation with spheres - 82
A. Aggregation method
B. Injection method
5.2.2 Genotyping - 82
5.2.3 Immunohistological analyses - 82
5.3 Results - 83
5.3.1 Generation of chimera zygotes - 83
5.3.2 アnalyses of adult chimera mice - 83
5.3.3 アnalyses of chimera fetuses - 83
5.4 Summary of section 5 - 84
5.5 Discussion - 85
5.6 References - 93
6. Future prospects
6.1 General overview - 95
6.2 Cell source for tissue engineering regenerative medicine - 95
6.3 Future issues - 96
APPENDIX - 97
• Material and method
• Primer sequences
ACKNOWLEDGEMENTS
CURRICULUM VITAE
(和訳)
目次
1.背景
1.1概論 - 1
•細胞の重要性
•すべての幹細胞のユニークな特性
1.2成体幹細胞 - 7
•成体幹細胞研究の歴史
・成体幹細胞の機能
1.3成体幹細胞を同定するための研究方法 - 10
•成体幹細胞の正常な分化経路
•分化転換。
1.4多能性幹細胞 - 14
•胚性および成体幹細胞の類似点と相違点
•胚性幹細胞
A.実験室で増殖した胚性幹細胞の樹立
B.胚性幹細胞の同定試験
・誘導性多能性幹細胞
1.5多能性成体幹細胞存在の可能性 - 20
•胞子様幹細胞
1.6この研究の仮説 - 23
2.小細胞の単離
2.1序論 - 27
2.1.1幹細胞と小細胞 - 27
2.1.2幹細胞と球体形成 - 27
2.2実験 - 28
2.2.1小細胞の分離 - 28
2.2.2小細胞の特性 - 29
2.3結果 - 30
2.3.1単離細胞からの球体形成 - 30
2.3.2トリチュレーション効果 - 30
2.3.3小細胞の培養条件 - 31
2.3.4骨髄細胞からの球体形成 - 31
2.3.5未熟細胞マーカー発現 - 32
2.3.6胚的表現型の発現 - 33
2.3.7多能性マーカーの定量PCR - 34
2.4第二章のまとめ - 35
2.5討論 - 35
2.6参考文献 - 45
3.小細胞の特性
3.1序論 - 48
3.1.1幹細胞の分化能
3.2実験 - 48
3.2.1試験管内での細胞の分化能 - 48
3.2.2生体内での分化能 - 48
3.3結果 - 49
3.3.1試験管内での細胞の分化能 - 50
3.3.2生体内での細胞の分化能 - 51
3.4第三章のまとめ - 51
3.5討論 - 51
3.6参照 - 58
4.全三胚葉由来組織からの小細胞の単離
4.1序論 - 63
4.2実験 - 63
4.2.1様々な組織からの小細胞の分離 - 64
4.2.2分離された小細胞の特性 - 64
4.2.3球体を形成する小細胞の分化能 - 64
4.3結果 - 65
4.3.1三胚葉代表由来組織からの球体形成 - 65
4.3.2三胚葉代表組織由来球体の未熟遺伝子発現 - 66
4.3.3三胚葉代表組織由来細胞の分化能 - 67
4.3.4生体内における細胞の分化能 - 68
4.4第四章のまとめ - 69
4.5討論 - 69
4.6参考文献 - 76
5.分離小細胞からのキメラマウス作製
5.1序論 - 81
5.1.1成体幹細胞とキメラマウス - 81
5.2実験 - 81
5.2.1球体によるキメラマウス作製 - 82
A.凝集法
B.注入法
5.2.2遺伝子型決定 - 82
5.2.3免疫組織学分析 - 82
5.3結果 - 83
5.3.1キメラ接合体の作製 - 83
5.3.2成長キメラマウスの分析 - 83
5.3.3キメラ胎児の分析-83
5.4第五章のまとめ - 84
5.5討論 - 85
5.6参考文献 - 93
今後の展望
6.1概観 - 95
6.2組織工学的再生医療用の細胞供給源 - 95
6.3今後の課題 - 96
付録 - 97
•材料と方法
•プライマー配列
謝辞
履歴書
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